Phospho-Flow Sample Data
Our 10 sample datasets are distributed into three categories listed below. These datasets exemplify
the power of flow cytometric analysis of phospho-epitopes. We strongly
recommend that first-time users start with cell lines
and one or two color intracellular phospho-stains before proceeding
to multi-color stains with surface markers. Those wishing to work on
human or murine samples with surface markers
are encouraged to visit the protocols page for further information.
- Good introduction to the type of
data produced by these approaches. Those planning on using
flow cytometry to investigate intracellular signaling are encouraged
to begin by repeating one or more experiments presented in this category.
- The technique is applied to mixed populations of cells such as
the lymphoid compartments of humans and mice.
- Demonstrates that phospho-epitope analysis by
an 11 color flow cytometer can reveal functional specificities not
conveyed by other approaches.
